Recombinant:Article Title: Ancient genomes provide insight into the Paleolithic-to-Neolithic transition in northern East Asia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ancient skeletal element This study DHL_M1 Ancient skeletal element This study DHL_M2 Ancient skeletal element This study DHL_M02 Chemicals, peptides, and recombinant proteins Water, HPLC-grade Merck cat. no. 270733 5 M NaCl solution Merck cat. no. S5150-1L 1 M Tris-HCl, pH 8.0 AppliChem A4577,0500 0.5 M EDTA, pH 8.0 AppliChem A4892,1000 Tween-20 Merck cat. no. P5927-100ML 20x SSC buffer Thermo Fisher Scientific cat. no. AM9763 20% (wt/vol) SDS solution Thermo Fisher Scientific cat. no. AM9820 3 M Sodium acetate, pH 5.2 Merck cat. no. S7899-500ML T4 RNA ligase reaction buffer, including 50% (wt/vol) PEG 8000 NEB cat. no. B0216L ATP solution (100 mM) Thermo Fisher Scientific cat. no. R0441 Klenow fragment, including 10x reaction buffer Thermo Fisher Scientific cat. no. EP0052 T4 polynucleotide kinase 10 U/μl Thermo Fisher Scientific cat. no. EK0031 FastAP thermosensitive alkaline phosphatase Thermo Fisher Scientific cat. no. EF0651 T4 DNA ligase, high concentrated 30 U/μl Thermo Fisher Scientific cat. no. EL0013 T4 DNA ligase, 5 U/μl, including 10x reaction buffer and 50% (wt/vol) PEG-4000 Thermo Fisher Scientific cat. no. EL0012 Herculase II fusion DNA polymerase, including 5x Herculase II reaction buffer Agilent cat. no.600679 Dynabeads MyOne Streptavidin C1 Life Technologies cat. no. 65001 25 mM each dNTP Thermo Fisher Scientific cat. no. R1121 Maxima Probe qPCR Master Mix (2x) Thermo Fisher Scientific cat. no. K0261 AccuPrime Pfx DNA polymerase Thermo Fisher Scientific cat. no. 12344-024 MinElute PCR Purification Kit Qiagen cat. no. 28006 Agilent DNA 1000 Kit Agilent cat. no. 5067-1504 Cot-1 DNA Invitrogen 15279011 2x Hi-RPM hybridization buffer Agilent 5118–5380 AmpliTaq Gold 10x PCR buffer without Mg Life Technologies 4379874 1M NaOH Sigma 71463–1L SeraMag Speedbeads GE 65152105050250 Deposited data The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive54 in National Genomics Data Center,55 China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences that are publicly accessible at https://ngdc.cncb.ac.cn/gsa-human. ..
High Performance Liquid Chromatography:Article Title: Ancient genomes provide insight into the Paleolithic-to-Neolithic transition in northern East Asia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ancient skeletal element This study DHL_M1 Ancient skeletal element This study DHL_M2 Ancient skeletal element This study DHL_M02 Chemicals, peptides, and recombinant proteins Water, HPLC-grade Merck cat. no. 270733 5 M NaCl solution Merck cat. no. S5150-1L 1 M Tris-HCl, pH 8.0 AppliChem A4577,0500 0.5 M EDTA, pH 8.0 AppliChem A4892,1000 Tween-20 Merck cat. no. P5927-100ML 20x SSC buffer Thermo Fisher Scientific cat. no. AM9763 20% (wt/vol) SDS solution Thermo Fisher Scientific cat. no. AM9820 3 M Sodium acetate, pH 5.2 Merck cat. no. S7899-500ML T4 RNA ligase reaction buffer, including 50% (wt/vol) PEG 8000 NEB cat. no. B0216L ATP solution (100 mM) Thermo Fisher Scientific cat. no. R0441 Klenow fragment, including 10x reaction buffer Thermo Fisher Scientific cat. no. EP0052 T4 polynucleotide kinase 10 U/μl Thermo Fisher Scientific cat. no. EK0031 FastAP thermosensitive alkaline phosphatase Thermo Fisher Scientific cat. no. EF0651 T4 DNA ligase, high concentrated 30 U/μl Thermo Fisher Scientific cat. no. EL0013 T4 DNA ligase, 5 U/μl, including 10x reaction buffer and 50% (wt/vol) PEG-4000 Thermo Fisher Scientific cat. no. EL0012 Herculase II fusion DNA polymerase, including 5x Herculase II reaction buffer Agilent cat. no.600679 Dynabeads MyOne Streptavidin C1 Life Technologies cat. no. 65001 25 mM each dNTP Thermo Fisher Scientific cat. no. R1121 Maxima Probe qPCR Master Mix (2x) Thermo Fisher Scientific cat. no. K0261 AccuPrime Pfx DNA polymerase Thermo Fisher Scientific cat. no. 12344-024 MinElute PCR Purification Kit Qiagen cat. no. 28006 Agilent DNA 1000 Kit Agilent cat. no. 5067-1504 Cot-1 DNA Invitrogen 15279011 2x Hi-RPM hybridization buffer Agilent 5118–5380 AmpliTaq Gold 10x PCR buffer without Mg Life Technologies 4379874 1M NaOH Sigma 71463–1L SeraMag Speedbeads GE 65152105050250 Deposited data The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive54 in National Genomics Data Center,55 China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences that are publicly accessible at https://ngdc.cncb.ac.cn/gsa-human. ..
Real-time Polymerase Chain Reaction:Article Title: Ancient genomes provide insight into the Paleolithic-to-Neolithic transition in northern East Asia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ancient skeletal element This study DHL_M1 Ancient skeletal element This study DHL_M2 Ancient skeletal element This study DHL_M02 Chemicals, peptides, and recombinant proteins Water, HPLC-grade Merck cat. no. 270733 5 M NaCl solution Merck cat. no. S5150-1L 1 M Tris-HCl, pH 8.0 AppliChem A4577,0500 0.5 M EDTA, pH 8.0 AppliChem A4892,1000 Tween-20 Merck cat. no. P5927-100ML 20x SSC buffer Thermo Fisher Scientific cat. no. AM9763 20% (wt/vol) SDS solution Thermo Fisher Scientific cat. no. AM9820 3 M Sodium acetate, pH 5.2 Merck cat. no. S7899-500ML T4 RNA ligase reaction buffer, including 50% (wt/vol) PEG 8000 NEB cat. no. B0216L ATP solution (100 mM) Thermo Fisher Scientific cat. no. R0441 Klenow fragment, including 10x reaction buffer Thermo Fisher Scientific cat. no. EP0052 T4 polynucleotide kinase 10 U/μl Thermo Fisher Scientific cat. no. EK0031 FastAP thermosensitive alkaline phosphatase Thermo Fisher Scientific cat. no. EF0651 T4 DNA ligase, high concentrated 30 U/μl Thermo Fisher Scientific cat. no. EL0013 T4 DNA ligase, 5 U/μl, including 10x reaction buffer and 50% (wt/vol) PEG-4000 Thermo Fisher Scientific cat. no. EL0012 Herculase II fusion DNA polymerase, including 5x Herculase II reaction buffer Agilent cat. no.600679 Dynabeads MyOne Streptavidin C1 Life Technologies cat. no. 65001 25 mM each dNTP Thermo Fisher Scientific cat. no. R1121 Maxima Probe qPCR Master Mix (2x) Thermo Fisher Scientific cat. no. K0261 AccuPrime Pfx DNA polymerase Thermo Fisher Scientific cat. no. 12344-024 MinElute PCR Purification Kit Qiagen cat. no. 28006 Agilent DNA 1000 Kit Agilent cat. no. 5067-1504 Cot-1 DNA Invitrogen 15279011 2x Hi-RPM hybridization buffer Agilent 5118–5380 AmpliTaq Gold 10x PCR buffer without Mg Life Technologies 4379874 1M NaOH Sigma 71463–1L SeraMag Speedbeads GE 65152105050250 Deposited data The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive54 in National Genomics Data Center,55 China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences that are publicly accessible at https://ngdc.cncb.ac.cn/gsa-human. ..
Polymerase Chain Reaction:Article Title: Ancient genomes provide insight into the Paleolithic-to-Neolithic transition in northern East Asia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ancient skeletal element This study DHL_M1 Ancient skeletal element This study DHL_M2 Ancient skeletal element This study DHL_M02 Chemicals, peptides, and recombinant proteins Water, HPLC-grade Merck cat. no. 270733 5 M NaCl solution Merck cat. no. S5150-1L 1 M Tris-HCl, pH 8.0 AppliChem A4577,0500 0.5 M EDTA, pH 8.0 AppliChem A4892,1000 Tween-20 Merck cat. no. P5927-100ML 20x SSC buffer Thermo Fisher Scientific cat. no. AM9763 20% (wt/vol) SDS solution Thermo Fisher Scientific cat. no. AM9820 3 M Sodium acetate, pH 5.2 Merck cat. no. S7899-500ML T4 RNA ligase reaction buffer, including 50% (wt/vol) PEG 8000 NEB cat. no. B0216L ATP solution (100 mM) Thermo Fisher Scientific cat. no. R0441 Klenow fragment, including 10x reaction buffer Thermo Fisher Scientific cat. no. EP0052 T4 polynucleotide kinase 10 U/μl Thermo Fisher Scientific cat. no. EK0031 FastAP thermosensitive alkaline phosphatase Thermo Fisher Scientific cat. no. EF0651 T4 DNA ligase, high concentrated 30 U/μl Thermo Fisher Scientific cat. no. EL0013 T4 DNA ligase, 5 U/μl, including 10x reaction buffer and 50% (wt/vol) PEG-4000 Thermo Fisher Scientific cat. no. EL0012 Herculase II fusion DNA polymerase, including 5x Herculase II reaction buffer Agilent cat. no.600679 Dynabeads MyOne Streptavidin C1 Life Technologies cat. no. 65001 25 mM each dNTP Thermo Fisher Scientific cat. no. R1121 Maxima Probe qPCR Master Mix (2x) Thermo Fisher Scientific cat. no. K0261 AccuPrime Pfx DNA polymerase Thermo Fisher Scientific cat. no. 12344-024 MinElute PCR Purification Kit Qiagen cat. no. 28006 Agilent DNA 1000 Kit Agilent cat. no. 5067-1504 Cot-1 DNA Invitrogen 15279011 2x Hi-RPM hybridization buffer Agilent 5118–5380 AmpliTaq Gold 10x PCR buffer without Mg Life Technologies 4379874 1M NaOH Sigma 71463–1L SeraMag Speedbeads GE 65152105050250 Deposited data The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive54 in National Genomics Data Center,55 China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences that are publicly accessible at https://ngdc.cncb.ac.cn/gsa-human. ..
Purification:Article Title: Ancient genomes provide insight into the Paleolithic-to-Neolithic transition in northern East Asia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ancient skeletal element This study DHL_M1 Ancient skeletal element This study DHL_M2 Ancient skeletal element This study DHL_M02 Chemicals, peptides, and recombinant proteins Water, HPLC-grade Merck cat. no. 270733 5 M NaCl solution Merck cat. no. S5150-1L 1 M Tris-HCl, pH 8.0 AppliChem A4577,0500 0.5 M EDTA, pH 8.0 AppliChem A4892,1000 Tween-20 Merck cat. no. P5927-100ML 20x SSC buffer Thermo Fisher Scientific cat. no. AM9763 20% (wt/vol) SDS solution Thermo Fisher Scientific cat. no. AM9820 3 M Sodium acetate, pH 5.2 Merck cat. no. S7899-500ML T4 RNA ligase reaction buffer, including 50% (wt/vol) PEG 8000 NEB cat. no. B0216L ATP solution (100 mM) Thermo Fisher Scientific cat. no. R0441 Klenow fragment, including 10x reaction buffer Thermo Fisher Scientific cat. no. EP0052 T4 polynucleotide kinase 10 U/μl Thermo Fisher Scientific cat. no. EK0031 FastAP thermosensitive alkaline phosphatase Thermo Fisher Scientific cat. no. EF0651 T4 DNA ligase, high concentrated 30 U/μl Thermo Fisher Scientific cat. no. EL0013 T4 DNA ligase, 5 U/μl, including 10x reaction buffer and 50% (wt/vol) PEG-4000 Thermo Fisher Scientific cat. no. EL0012 Herculase II fusion DNA polymerase, including 5x Herculase II reaction buffer Agilent cat. no.600679 Dynabeads MyOne Streptavidin C1 Life Technologies cat. no. 65001 25 mM each dNTP Thermo Fisher Scientific cat. no. R1121 Maxima Probe qPCR Master Mix (2x) Thermo Fisher Scientific cat. no. K0261 AccuPrime Pfx DNA polymerase Thermo Fisher Scientific cat. no. 12344-024 MinElute PCR Purification Kit Qiagen cat. no. 28006 Agilent DNA 1000 Kit Agilent cat. no. 5067-1504 Cot-1 DNA Invitrogen 15279011 2x Hi-RPM hybridization buffer Agilent 5118–5380 AmpliTaq Gold 10x PCR buffer without Mg Life Technologies 4379874 1M NaOH Sigma 71463–1L SeraMag Speedbeads GE 65152105050250 Deposited data The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive54 in National Genomics Data Center,55 China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences that are publicly accessible at https://ngdc.cncb.ac.cn/gsa-human. ..
Hybridization:Article Title: Ancient genomes provide insight into the Paleolithic-to-Neolithic transition in northern East Asia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ancient skeletal element This study DHL_M1 Ancient skeletal element This study DHL_M2 Ancient skeletal element This study DHL_M02 Chemicals, peptides, and recombinant proteins Water, HPLC-grade Merck cat. no. 270733 5 M NaCl solution Merck cat. no. S5150-1L 1 M Tris-HCl, pH 8.0 AppliChem A4577,0500 0.5 M EDTA, pH 8.0 AppliChem A4892,1000 Tween-20 Merck cat. no. P5927-100ML 20x SSC buffer Thermo Fisher Scientific cat. no. AM9763 20% (wt/vol) SDS solution Thermo Fisher Scientific cat. no. AM9820 3 M Sodium acetate, pH 5.2 Merck cat. no. S7899-500ML T4 RNA ligase reaction buffer, including 50% (wt/vol) PEG 8000 NEB cat. no. B0216L ATP solution (100 mM) Thermo Fisher Scientific cat. no. R0441 Klenow fragment, including 10x reaction buffer Thermo Fisher Scientific cat. no. EP0052 T4 polynucleotide kinase 10 U/μl Thermo Fisher Scientific cat. no. EK0031 FastAP thermosensitive alkaline phosphatase Thermo Fisher Scientific cat. no. EF0651 T4 DNA ligase, high concentrated 30 U/μl Thermo Fisher Scientific cat. no. EL0013 T4 DNA ligase, 5 U/μl, including 10x reaction buffer and 50% (wt/vol) PEG-4000 Thermo Fisher Scientific cat. no. EL0012 Herculase II fusion DNA polymerase, including 5x Herculase II reaction buffer Agilent cat. no.600679 Dynabeads MyOne Streptavidin C1 Life Technologies cat. no. 65001 25 mM each dNTP Thermo Fisher Scientific cat. no. R1121 Maxima Probe qPCR Master Mix (2x) Thermo Fisher Scientific cat. no. K0261 AccuPrime Pfx DNA polymerase Thermo Fisher Scientific cat. no. 12344-024 MinElute PCR Purification Kit Qiagen cat. no. 28006 Agilent DNA 1000 Kit Agilent cat. no. 5067-1504 Cot-1 DNA Invitrogen 15279011 2x Hi-RPM hybridization buffer Agilent 5118–5380 AmpliTaq Gold 10x PCR buffer without Mg Life Technologies 4379874 1M NaOH Sigma 71463–1L SeraMag Speedbeads GE 65152105050250 Deposited data The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive54 in National Genomics Data Center,55 China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences that are publicly accessible at https://ngdc.cncb.ac.cn/gsa-human. ..
Sequencing:Article Title: Ancient genomes provide insight into the Paleolithic-to-Neolithic transition in northern East Asia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ancient skeletal element This study DHL_M1 Ancient skeletal element This study DHL_M2 Ancient skeletal element This study DHL_M02 Chemicals, peptides, and recombinant proteins Water, HPLC-grade Merck cat. no. 270733 5 M NaCl solution Merck cat. no. S5150-1L 1 M Tris-HCl, pH 8.0 AppliChem A4577,0500 0.5 M EDTA, pH 8.0 AppliChem A4892,1000 Tween-20 Merck cat. no. P5927-100ML 20x SSC buffer Thermo Fisher Scientific cat. no. AM9763 20% (wt/vol) SDS solution Thermo Fisher Scientific cat. no. AM9820 3 M Sodium acetate, pH 5.2 Merck cat. no. S7899-500ML T4 RNA ligase reaction buffer, including 50% (wt/vol) PEG 8000 NEB cat. no. B0216L ATP solution (100 mM) Thermo Fisher Scientific cat. no. R0441 Klenow fragment, including 10x reaction buffer Thermo Fisher Scientific cat. no. EP0052 T4 polynucleotide kinase 10 U/μl Thermo Fisher Scientific cat. no. EK0031 FastAP thermosensitive alkaline phosphatase Thermo Fisher Scientific cat. no. EF0651 T4 DNA ligase, high concentrated 30 U/μl Thermo Fisher Scientific cat. no. EL0013 T4 DNA ligase, 5 U/μl, including 10x reaction buffer and 50% (wt/vol) PEG-4000 Thermo Fisher Scientific cat. no. EL0012 Herculase II fusion DNA polymerase, including 5x Herculase II reaction buffer Agilent cat. no.600679 Dynabeads MyOne Streptavidin C1 Life Technologies cat. no. 65001 25 mM each dNTP Thermo Fisher Scientific cat. no. R1121 Maxima Probe qPCR Master Mix (2x) Thermo Fisher Scientific cat. no. K0261 AccuPrime Pfx DNA polymerase Thermo Fisher Scientific cat. no. 12344-024 MinElute PCR Purification Kit Qiagen cat. no. 28006 Agilent DNA 1000 Kit Agilent cat. no. 5067-1504 Cot-1 DNA Invitrogen 15279011 2x Hi-RPM hybridization buffer Agilent 5118–5380 AmpliTaq Gold 10x PCR buffer without Mg Life Technologies 4379874 1M NaOH Sigma 71463–1L SeraMag Speedbeads GE 65152105050250 Deposited data The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive54 in National Genomics Data Center,55 China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences that are publicly accessible at https://ngdc.cncb.ac.cn/gsa-human. ..
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